Cytoskeleton: What Does GTP Do for Septins?
نویسندگان
چکیده
The recent observation of GTP-promoted polymerization of a single septin polypeptide suggests that this protein has tubulin-like biochemical properties. This model cannot, however, explain the GTP-biochemistry of heteromeric septin complexes from cytosol.
منابع مشابه
Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4
Assembly at the mother-bud neck of a filamentous collar containing five septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1) is necessary for proper morphogenesis and cytokinesis. We show that Cdc10 and Cdc12 possess GTPase activity and appropriate mutations in conserved nucleotide-binding residues abrogate GTP binding and/or hydrolysis in vitro. In vivo, mutants unable to bind GTP prevent septin coll...
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ورودعنوان ژورنال:
- Current Biology
دوره 12 شماره
صفحات -
تاریخ انتشار 2002